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Sep 6, 2012 · Prior to polymerase binding, the incoming nucleotide is complexed with divalent magnesium. Magnesium can bind to any of the phosphates forming ...
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Here, we present a crystal structure of a precatalytic complex of a DNA polymerase with bound substrates that include the primer 3'-OH and catalytic Mg2+.
Here, we present a crystal structure of a precatalytic complex of a DNA polymerase with bound substrates that include the primer 3′-OH and catalytic Mg2+. This ...
Feb 20, 2009 · It arises from capture of binary or ternary complex, with the duplex DNA held atop the pore vestibule because of association with KF. (ii) The ...
Missing: Magnesium | Show results with:Magnesium
Feb 14, 2024 · Thermodynamic coupling constants between the DNA and nucleotide binding states reveal binding linkage within the RecBCD•DNA•nucleotide complex.
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Subsequently, the second Mg2+ binds to the closed state of the enzyme–DNA–Mg.dNTP complex (Kd = 3.7 mM) to facilitate catalysis. Weak binding of the catalytic ...
Jul 6, 2017 · In this study, the binding between Dbh and DNA was investigated to better understand how to rationally design enhanced processivity in a Y- ...
Abstract. Binding of the catalytic divalent ion to the ternary DNA polymerase β/gapped DNA/dNTP complex is thought to represent the final step in the assem.
We report here structures of human AGT in complex with double-stranded DNA containing the biological substrate O(6)-methylguanine or crosslinked to the ...
Jun 29, 2023 · Repair of topoisomerase 1–induced DNA damage by tyrosyl-DNA phosphodiesterase 2 (TDP2) is dependent on its magnesium binding. Naoto Shimizu.